Documentation · v0.3.0

Everything you need
to trust the fit.

Guides, references, and walkthroughs for the assays Gradiance runs — written by the bioengineers who built it.

· Quick start ·· 4PL / 5PL fitting ·· ELISA standard curves ·· qPCR ΔΔCt ·· Quality flags ·· Reproducible exports ·· Statistical methods ·· How to cite ·
· Quick start ·· 4PL / 5PL fitting ·· ELISA standard curves ·· qPCR ΔΔCt ·· Quality flags ·· Reproducible exports ·· Statistical methods ·· How to cite ·

Watch it work

A real BCA run,
recorded.

Drop in a 9-row absorbance plate, pick the BCA workflow, hit start. Gradiance fits an 8-point BSA standard curve, back-calculates the unknowns, and writes a one-paragraph interpretation — typically in under twenty seconds.

8-pt
BSA standards
R² 0.999
quadratic fit
<20 s
end-to-end
gradiance.io / runs / #zrel8f76live
Recording of a live BCA / Bradford analysis run from upload to result

Captured live · run #zrel8f76 · 8-point BSA standard, 8 unknowns, R² = 0.999.

The tour

The screenshots, before the words.

These are real screens of the running app — not mockups. Skim them, then dive into the guides below.

gradiance.io
A 4PL fit, with everything you'd cite.

Frame · 01 / 02 · Output

A 4PL fit, with everything you'd cite.

Curve, residuals, CIs on every parameter, model equation, plain-English interpretation, and QC checks — all in one viewer. Switch palettes, copy the equation, or download the underlying CSV.

Read the dose-response guide
gradiance.io
qPCR ΔΔCt across a panel — same viewer.

Frame · 02 / 02 · Multi-fit

qPCR ΔΔCt across a panel — same viewer.

Fold-change for each target, propagated error, and significance flags. The same panel handles ELISA standards, kinetics, and binding too — every assay rendered with the same trustworthy chrome.

See standard curves

Postscript

Still stuck?
Write to us.

The docs miss things. The team doesn't. Real humans, usually within a day.

Built by PhDs
~24h response
UPenn + Northwestern