Quick Start Guide

Sign In

Navigate to the Gradiance homepage and sign in with your Google account. No additional setup required.

Create a New Run

• Click the "New Run" button in the sidebar
• You'll be taken to a new analysis workspace
• The workspace has three main areas: file upload, AI assistant, and results panel

Upload Your Data

Gradiance accepts CSV and Excel files with your dose-response data. Your file should contain:

• Concentration column: The drug/compound concentrations tested
• Response column: The measured response (e.g., % viability, absorbance)

Concentration,Response
0.001,95.2
0.01,92.1
0.1,78.5
1,45.3
10,12.8
100,5.1

Tell the AI What You Want

In the AI assistant panel, describe your analysis in natural language. For example:

"Please analyze this dose-response data and calculate the IC50 using a 4-parameter logistic fit."

The AI will:

• Read your uploaded file
• Detect concentration and response columns
• Fit a 4PL curve to your data
• Calculate IC₅₀, R², and other parameters
• Generate a publication-quality plot

Review Your Results

The results panel will display:

• Interactive plot: Your fitted curve with data points
• Parameters: IC₅₀, Hill slope, R²
• QC warnings: Any quality control flags (e.g., low R²)

Refine Your Analysis

You can ask follow-up questions to refine your analysis:

• "Try a 5PL fit instead"
• "Exclude the outlier at 100 µM"
• "Constrain the bottom to 0"
• "Show me the residual plot"

Export Your Results

Once your analysis is complete, use the Export dropdown in the workspace header to download your results in any supported format:

• Plot (PNG, 300 DPI) — publication-ready raster image
• Plot (SVG) — scalable vector graphic for editing
• Report (PDF) — full analysis report with plot and parameters
• Results (CSV) — fitted values and calculated parameters
• Data (Excel) — input and output data in a spreadsheet
• Python Script — reproducible script that regenerates your analysis