Getting Started
Understanding Your Results
Gradiance provides comprehensive output metrics to help you interpret your experiment.
Key Parameters
- IC₅₀ / EC₅₀
- The concentration of agonist that gives a response half way between Bottom and Top. This is the primary measure of potency.
- R-Squared (R²)
- Quantifies goodness of fit. An R² of 0.99 means the model explains 99% of the variance in the data.
- Hill Slope
- Describes the steepness of the curve. A standard slope is 1.0. Values significantly greater than 1 suggest cooperativity.
- Span
- The absolute difference between the Top and Bottom plateaus. Small spans indicate a weak signal window.
Confidence Intervals
We provide 95% Confidence Intervals (CI) for every parameter. If the CI is very wide (e.g., spans orders of magnitude), it means the parameter could not be determined with precision, usually because the data does not fully define the curve (e.g., incomplete saturation).